Regulatory

Part:BBa_K2771003

Designed by: Felipe Xavier Buson   Group: iGEM18_USP-Brazil   (2018-10-06)


Modified pRpa

Quorum sensing activated promoter that responds natively to the Rpa pathway (activated by RpaR + pCHSL). This specific instance of this promoter has a diversity of differences to the wild-type promoter, sharing a consensus between bases 2 and 16. The sequence was optimised by SR. Scott and J. Hasty (doi: 10.1021/acssynbio.5b00286) to show diminished crosstalk with the Lux, Las and Tra systems.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None